Pathogenic instruction of CD8 T cell differentiation in Crohn’s disease

Project Summary

This project will continue to investigate the mechanisms of impaired induction of T cell exhaustion in Crohn’s disease (CD). A03 will generate single-cell transcriptomic and epigenomic datasets in active disease and remission and use integrated bioinformatics to uncover and test potential regulators of differentiation. TCR analysis will be performed on the identified disease-related CD8 T cell populations to understand if clonal expansion suggesting recognition of specific CD-related antigens is involved. A03 will also analyze spatial interaction partners of these cells using highly multiplexed imaging mass cytometry.